Digital Measurements: A Different Approach to Evaluate Bone Formation

Autor:	M. Gordjestani, Luc Dermaut, F. Bosman, P. De Waele, L. De Ridder, W. De Leersnijder
Rok vydání:	2006
Předmět:	Pathology medicine.medical_specialty Histology biology business.industry Digital imaging Anatomy Bone remodeling biology.protein Medicine Bone formation Osteopontin business
Zdroj:	Cells Tissues Organs. 184:148-153
ISSN:	1422-6421 1422-6405
DOI:	10.1159/000099621
Popis:	In this study a digital measurement technique has been proposed to quantify bone formation on histological images. Two standard parietal defects were created in 30 adult rabbits. The animals were divided into six groups. Four animals of each group were randomly chosen as experimental group in which osteopontin-coated hydroxyapatite (OPN-HA) and hydroxyapatite (HA) were inserted alternatively in created defects. To observe the spontaneous healing process of defects, one animal of each group was used as control animal and these created defects did not receive any implants. The animals were sacrificed after 1, 2, 6, 12, 18 and 30 weeks. The histological sections were magnified (×100) and scanned digitally. The newly formed bone surfaces within the healing area were indicated and quantified by means of Adobe Photoshop® 7 software. This measuring technique was found to be reliable and reproducible. The results of this study show no significant differences in bone formation between the OPN-HA and non-coated HA defects, although a significant difference in bone formation was measured at the margins of the defects treated with OPN-HA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::597ee6cbd8111529989b5a6d6b7522e2 https://doi.org/10.1159/000099621 Zobrazit plný text záznamu