Liquid Chromatographic Determination of Progestogens in Animal Fat

Autor:	Michael T Andresen, Adrian C E Fesser
Rok vydání:	1996
Předmět:	Pharmacology Detection limit Animal fat Chromatography Magnesium Elution Extraction (chemistry) chemistry.chemical_element Analytical Chemistry Hexane chemistry.chemical_compound chemistry Sodium hydroxide Environmental Chemistry Agronomy and Crop Science Saponification Food Science
Zdroj:	Journal of AOAC INTERNATIONAL. 79:1037-1042
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/79.5.1037
Popis:	A liquid chromatographic method was developed for determination of 3 progestogens�melengestrol acetate, megestrol acetate, and chlormadinone acetate� found in edible tissue at concentrations between 10 and 1000 ppb. These progestogens are commonly used as feed additives to control herd estrus and to improve feed efficiency. Rendered fat was extracted with acetonitrile, washed with hexane, and dried. The remaining lipids were saponified with sodium hydroxide and precipitated with magnesium chloride. The progestogens were extracted from the basic solution with hexane, dried, and cleaned up on a cyanopropyl solid-phase extraction column in the normal-phase mode. The eluate was dried and reconstituted with acetonitrilewater (7 + 3, v/v). Chromatography was performed on a 5 μm high-carbon load C18 column with acetonitrile- water (7 + 3, v/v) at 1 mL/min and UV detection at 291 nm. Recoveries from fortified samples ranged from 84 to 116%. The limit of quantitation was 10 ppb for both beef and pork. The detection limit was 3 ppb.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5973793a44df516c034ea54ad35c7502 https://doi.org/10.1093/jaoac/79.5.1037 Zobrazit plný text záznamu