A novel hemA mutation is responsible for a small-colony-variant phenotype in Escherichia coli

Autor:	Adam P. Roberts, Alasdair T. M. Hubbard, Issra Bulgasim
Rok vydání:	2021
Předmět:	Mutation Chemistry Auxotrophy Biofilm Reductase medicine.disease_cause Microbiology Phenotype chemistry.chemical_compound medicine Gene Escherichia coli Hemin
Zdroj:	Microbiology. 167
ISSN:	1465-2080 1350-0872
Popis:	We identified a small colony variant (SCV) of an amoxicillin/clavulanic acid-resistant derivative of a clinical isolate of Escherichia coli from Malawi, which was selected for in vitro in a subinhibitory concentration of gentamicin. The SCV was auxotrophic for hemin and had impaired biofilm formation compared to the ancestral isolates. A single novel nucleotide polymorphism (SNP) in hemA, which encodes a glutamyl-tRNA reductase that catalyses the initial step of porphyrin biosynthesis leading to the production of haem, was responsible for the SCV phenotype. We showed the SNP in hemA resulted in a significant fitness cost to the isolate, which persisted even in the presence of hemin. However, the phenotype quickly reverted during sequential sub-culturing in liquid growth media. As hemA is not found in mammalian cells, and disruption of the gene results in a significant fitness cost, it represents a potential target for novel drug development specifically for the treatment of catheter-associated urinary tract infections caused by E. coli .
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::59620214514cfd74650a191a3fcaa2a2 https://doi.org/10.1099/mic.0.000962 Zobrazit plný text záznamu