HPV Typing by in Situ Hybridization on Cervical Cytologic Smears with ASCUS
| Autor: | Raymond Seite, Martine Joannes, Amapola Autillo-Touati, Andrée Robaglia-Schlupp, Alain Lambert, Bernard Blanc, Claude D'Ercole, Evelyne Mazzella |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pathology
medicine.medical_specialty Histology business.industry Hybridization probe virus diseases General Medicine In situ hybridization Virology female genital diseases and pregnancy complications Koilocyte Pathology and Forensic Medicine Cytopathology Genotype medicine Typing Cervix neoplasm business Ascus |
| Zdroj: | Acta Cytologica. 42:631-638 |
| ISSN: | 1938-2650 0001-5547 |
| Popis: | OBJECTIVE: To assess the prognostic significance of atypical squamous cells of undetermined significance (ASCUS) using an in situ hybridization (ISH) method for destained cervical cytologic smears and a cocktail of biotinylated DNA probes for human papillomavirus (HPV) 6, 11, 16, 18, 31 and 33. STUDY DESIGN: Two HPV DNA probe mixtures were applied to the same smear for the simultaneous detection of high-risk HPV types 16, 18, 31 and 33 and low-risk HPV 6 and 11. ISH was carried out on 192 smears. Among them, 59 showed koilocytosis, 91 ASCUS and 42 normal features. RESULTS: Low-risk HPV types were rarely found and associated mainly with koilocytosis (17%). High rates of potentially oncogenic HPV were detected in ASCUS (41%) and condyloma (73%). In addition, similar levels of positivity were found to be associated with ASCUS when using two probe mixtures specific to high-risk HPV: one included HPV genotypes 16 and 18 and the other, genotypes 31 and 33. CONCLUSION: HPV DNA typing by ISH on cervical cytologic smears might improve the identification of women at high risk of developing precancerous and cancerous cervical lesions. |
| Databáze: | OpenAIRE |
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