Allopurinol supplementation of the growth medium enhances the fermentation of lignocellulosic hydrolysates to ethanol by Saccharomyces cerevisiae
| Autor: | Thaddeus Chukwuemeka Ezeji, Chidozie Victor Agu, Victor Ujor |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Growth medium Ethanol biology Saccharomyces cerevisiae Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Hydrolysate 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Corn stover chemistry Nucleic acid biosynthesis Ethanol fuel Fermentation Food science Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 14:151-159 |
| ISSN: | 1878-8181 |
| DOI: | 10.1016/j.bcab.2018.03.001 |
| Popis: | To fully harness lignocellulosic sugars as fermentation substrates for the production of biofuels and fine chemicals, the tolerance of fermenting microbes to lignocellulose-derived microbial inhibitory compounds (LDMICs) must be improved. Towards this goal, we exploited the ability of allopurinol, a xanthine dehydrogenase/oxidase inhibitor, to promote purine salvage and nucleic acid biosynthesis to alleviate LDMIC-induced DNA damage, thereby improving the LDMIC tolerance of Saccharomyces cerevisiae NRRL Y-566 during the fermentation of lignocellulosic sugars to ethanol. By supplementing LDMIC-replete wheat straw and corn stover hydrolysates (CSH) with 0.5–8 mM allopurinol, a concentration-dependent increase in ethanol production was observed. Also, by pulse-feeding allopurinol to a model sugar-LDMIC mixture and switching fermentation from aerobic to microaerophilic condition at the exponential growth phase of S. cerevisiae NRRL Y-566, cell growth, ethanol concentration, productivity, and yield improved by 12.0%, 53.1%, 54.0%, and 50.0%, respectively, when compared to the allopurinol-unsupplemented control. Similarly, when 100% (v/v) CSH was used as carbon source, S. cerevisiae NRRL Y-566 growth, ethanol titer, productivity, and yield increased by 19%, 21%, 20% and 24%, respectively. Additionally, allopurinol reduced the onset of stationary growth phase of S. cerevisiae by 12 h and extended its chronological lifespan by 16 h during growth in100% CSH. These results underscore the feasibility of exploiting allopurinol-mediated increase in LDMIC tolerance by S. cerevisiae for the fermentation of lignocellulosic-derived sugars to ethanol. |
| Databáze: | OpenAIRE |
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