Molecular sex identification of red panda (Ailurus fulgens) suitable for noninvasive genetic studies

Autor:	P. Anuradha Reddy, Bhupen Roka, Arun Kumar, Upashna Rai
Rok vydání:	2015
Předmět:	Genetics Sequence analysis In silico Sexing Management Monitoring Policy and Law Biology Molecular biology law.invention law Primer (molecular biology) Amelogenin Gene AMELX Ecology Evolution Behavior and Systematics Polymerase chain reaction Nature and Landscape Conservation
Zdroj:	European Journal of Wildlife Research. 61:641-644
ISSN:	1439-0574 1612-4642
DOI:	10.1007/s10344-015-0928-2
Popis:	Molecular sexing of wild animals is feasible with differential amplification of X- and Y-homologous regions of various genes. We present a simple and robust technique for sex identification of red panda suitable for noninvasive studies based on sex-specific fragment size polymorphism in amelogenin (AMEL) gene. Sequence analysis of AMELX and AMELY revealed an 18-bp deletion in Y fragment which was used to differentially amplify 217 bp of X and 199 bp of Y fragments. The designed primer pair is very sensitive and exhibits polymerase chain reaction (PCR) success rates of 89 and 78 % at 0.1 and 0.01 ng template DNA concentrations, respectively. Further, we amplified poor quality faecal DNA with 94 % success rate and average dropout rate of 15.6 %. In silico analysis shows that these primers can also be used to identify sex of a sympatric species, giant panda.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5958eade58907bd45844ff60594fc956 https://doi.org/10.1007/s10344-015-0928-2 Zobrazit plný text záznamu Full text from SpringerLink