Phanquinone: a useful fluorescent pre-chromatographic derivatization reagent for liquid chromatographic analyses of amino acid dosage forms

Autor:	Maria Grazia Gioia, A.M. Di Pietra, Rita Gatti
Rok vydání:	2002
Předmět:	chemistry.chemical_classification Chromatography Reversed-phase chromatography Biochemistry High-performance liquid chromatography Fluorescence Dosage form Analytical Chemistry Amino acid chemistry.chemical_compound chemistry Reagent Environmental Chemistry Phanquinone Derivatization Spectroscopy
Zdroj:	Analytica Chimica Acta. 474:11-20
ISSN:	0003-2670
DOI:	10.1016/s0003-2670(02)01011-5
Popis:	The use of 4,7-phenanthroline-5,6-dione (phanquinone) as a fluorogenic labeling reagent in pre-column derivatization for the LC separation of amino acids has been investigated. The compound, devoid of significant intrinsic fluorescence, reacts (60 min at 68 °C) with primary amino function to give fluorescent derivatives that can be separated by reversed-phase HPLC and detected at λex=400 nm, λem=460 nm. The experimental conditions for the derivatization and chromatographic separation are discussed. The proposed method proved to be suitable for the quality control of dosage forms (capsules, medicine in packets).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::59587ca75417688172d512d8e8b848d6 https://doi.org/10.1016/s0003-2670(02)01011-5 Zobrazit plný text záznamu Full Text from ScienceDirect