Popis: |
Background: In clinical practice, cartilage arthritis caused by cartilage tissue damage is difficult to treat due to the slow regeneration of cartilage tissue. In recent years, the orthopedic researches dedicate to promote the regeneration process. Recent reports reveal that appropriate shear force stimulation will exhibit an acceleration on proliferation and phenotypic differentiation of chondrocytes. At the same time, recent advances in 3D printing technology have made autologous cartilage tissue transplantation possible in clinical. Since the in vitro culture of autologous chondrocytes can provide a large amount of cell materials for clinical treatment, the optimization of in vitro culture conditions has become an urgent problem to be solved. For this reason, this study introduced an in vitro fluid shear force loading system and explored a set of optimal shear force parameters. Result: On the basis of these parameters, the in vitro proliferation speed and phenotypic differentiation level of chondrocytes are better than that of static culture. In addition, we used RNA interference to reduce the expression of ERK5 and proved that ERK5 is a key factor for chondrocytes to transmit shear force stimulation signals. Conclusion: These results have the opportunity to provide a theoretical basis for in vitro culture of chondrocytes and clinical treatment of cartilage injury. |