The Synthesis of Poly-L-Lysine-Succinyl-Nadp: An Analogue for Nadp/H
| Autor: | Wills Ra, Southard Wm, Sofer Ss |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Oxidase test
biology Hydrobromide Stereochemistry Lysine Biomedical Engineering Medicine (miscellaneous) Dimethylaniline chemistry.chemical_element Bioengineering General Medicine Oxygen Cofactor Biomaterials chemistry.chemical_compound chemistry biology.protein Microsome Nicotinamide adenine dinucleotide phosphate |
| Zdroj: | The International Journal of Artificial Organs. 3:293-297 |
| ISSN: | 1724-6040 0391-3988 |
| Popis: | Poly-L-lysine-succinyl-NADP, an analogue for nicotinamide adenine dinucleotide phosphate (NADP/H), has been synthesized by linking NADP/H to poly-L-lysine hydrobromide. This analogue (PL-SNP) was assayed with N, N dimethylaniline (DMA) in the presence of hepatic microsomal oxidase. A regeneration system of G-6-p and G-6-PD was used for reducing the PL-SNP+ to PL-SNPH. This reaction scheme with PL-SNP was found to exhibit up to 100% of the initial activity of pure NADP+ based on Polarographic studies. Reaction rate of production for dimethylaniline oxide (DMA-0) was determined by oxygen consumption and by oxide formation. One of the potential uses of this analogue is as a cofactor in a membrane/liver enzyme detoxification system. |
| Databáze: | OpenAIRE |
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