Cell-mediated Immunity against Allogeneic Tumor after in vitro Depletion of Histocompatibility Reactive Cells
| Autor: | Miodrag L. Lukic, Sidney Leskowitz |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
education.field_of_study
Population Cell Stimulus (physiology) Biology Molecular biology General Biochemistry Genetics and Molecular Biology In vitro Cell mediated immunity Histocompatibility chemistry.chemical_compound medicine.anatomical_structure chemistry Antigen Immunology medicine Thymidine education |
| Zdroj: | Experimental Biology and Medicine. 148:420-423 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | The “hot-pulse suicide” technique was first applied to lymphoid cells by Dutton and Mishell (1). The objective of this technique is to kill replicating cells in vitro by having them incorporate radioactive components such as thymidine, thereby eliminating the population proliferating in response to a specific antigen. Unidirectional mixed leukocyte cultures (MLC) “pulsed” with tri-tiated thymidine (3H-thy) of high specific radioactivity, for 3-36 hr 1 day after the initiation of the cultures were markedly inhibited in reactivity towards the stimulating cell but retained the capacity to respond to a second unrelated allogenic stimulus (2, 3). Similarly, MLC responses to a particular set of H-LA antigens can be suppressed by treatment with the thymidine analog 5-bro-modeoxyuridine (BUDR) followed by exposure to light in the visible or near visible region (4). This treatment does not significantly alter the capacity of the remaining lymphocytes to respond to cells of other H-LA specificity (4). Using the pop... |
| Databáze: | OpenAIRE |
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