Elicitor-Stimulated Furanocoumarin Biosynthesis in Cultured Parsley Cells: S-Adenosyl-ʟ-Methionine:Bergaptol and S-Adenosyl-ʟ-Methionine: Xanthotoxol O-Methyltransferases

Autor:	K. D. Hauffe, Klaus Hahlbrock, Dierk Scheel
Rok vydání:	1986
Předmět:	Isopimpinellin Methionine biology Ethionine food and beverages biology.organism_classification General Biochemistry Genetics and Molecular Biology Elicitor chemistry.chemical_compound chemistry Biochemistry Biosynthesis Phytophthora megasperma Xanthotoxol Petroselinum
Zdroj:	Zeitschrift für Naturforschung C. 41:228-239
ISSN:	1865-7125 0939-5075
DOI:	10.1515/znc-1986-1-234
Popis:	Cultured parsley cells (Petroselinum crispum) treated with an elicitor from the soybean patho­gen, Phytophthora megasperma f. sp. glycinea, were used as source for the separation, extensive purification and characterization of S-adenosyl-ʟ-methionine: xanthotoxol and S-adenosyl-ʟ-m ethionine: bergaptol O-methyltransferases. The products, xanthotoxin and bcrgapten, were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells. The latter enzyme also catalyzed the methylation of 5-hydroxyxanthotoxin to isopimpinellin, another major coumarin derivative in this system. The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products. No activity was detectable in untreated cells. Activity increases of the O-methyltransferases occurred a few hours later than those of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase.
Databáze:	OpenAIRE
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