Molecular identification of Shiitake [Lentinula edodes Berk (Pegler)] and production of secondary metabolites with biotechnological potential
| Autor: | Dagoberto Castro-Restrepo, Felipe Rojas-Rodas, Byron Duran-Rivera, Wilber Silva-López, Crhistian Gómez-Suárez |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology Epidemiology biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences 030104 developmental biology Infectious Diseases Lentinula Food science 010606 plant biology & botany Biotechnology Molecular identification |
| Zdroj: | Bionatura. 5:1183-1188 |
| ISSN: | 1390-9355 1390-9347 |
| DOI: | 10.21931/rb/2020.05.03.3 |
| Popis: | The Shitake mushroom (Lentinula edodes) is the second most-consumed mushroom in the world; in Colombia, it is cultivated and commercialized on a small scale in some supermarkets. Little is known about the precedence, nutritional and medicinal properties of Shiitake produced in Colombia. In this study, four shiitake isolates were grown in Colombia (LEUCO1, LEUCO2, LEUCO3, and LEUCO4) were sequenced in their ITS genes and evaluated for the production of three medicinal metabolites, eritadenine, ergotioneine and β-glucans (1,3-1, 6), using submerged culture. Genetic analysis revealed that all the isolates were close and related to the Japanese strain Cr62. LEUCO1 and LEUCO2 showed a distance of 0.000, as well as LEUCO3 and LEUCO4. All four isolates produced erythadenin in a range of 26.3-8.6 mg / L, with the best performance of LEUCO1 at 26.3 mg / L (p |
| Databáze: | OpenAIRE |
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