Characterization of Plasmodium falciparum-Infected Erythrocyte and P-Selectin Interaction Under Flow Conditions
| Autor: | Xiao-Fei Niu, Tineke Schollaardt, Sornchai Looareesuwan, May Ho, Paul Kubes, Kamala D. Patel |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
P-selectin
Cell adhesion molecule CD36 Immunology Plasmodium falciparum Cell Biology Hematology Adhesion Biology biology.organism_classification Biochemistry In vitro Sialic acid Cell biology chemistry.chemical_compound chemistry embryonic structures parasitic diseases biology.protein Platelet activation |
| Zdroj: | Blood. 91:4803-4809 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v91.12.4803 |
| Popis: | Plasmodium falciparum-infected erythrocytes (IRBC) roll on the adhesion molecule P-selectin in vitro under flow conditions that approximate the shear stress in capillary and postcapillary venules in which cytoadherence occurs in vivo. The pathological significance of this adhesive interaction is currently unknown. In this study, we further investigated the molecular interactions between IRBC and P-selectin by using a laminar flow system that allowed for the direct visualization of IRBC-substratum interactions. The results showed that the IRBC–P-selectin interaction was Ca2+-dependent and involved the lectin domain of P-selectin and a sialic acid residue on IRBC. The sialylated P-selectin ligand was trypsin-sensitive, which suggests that it could be part of the parasite antigen PfEMP1 that interacts with CD36 and intercellular adhesion molecule-1 (ICAM-1), but different from a trypsin-resistant IRBC ligand that adheres selectively to chondroitin sulfate A. Studies on the rolling and adhesion of IRBC on activated platelets that express both CD36 and P-selectin showed that inhibition of rolling on P-selectin reduced the adhesion of some clinical parasite isolates to CD36, whereas other parasite isolates appeared to interact directly with CD36. Thus, cytoadherence under physiological flow conditions may be mediated by multiple IRBC ligands that interact with different adhesion molecules in a cooperative fashion. These findings underscore the complexity of the interactions betweeen IRBC and vascular endothelium. |
| Databáze: | OpenAIRE |
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