Labslice XL – A Centrifugal Microfluidic Cartridge for the Automated Bio-Chemical Processing of Industrial Process Water
| Autor: | Roland Zengerle, Nils Paust, Lisa Drechsel, Felix von Stetten, Ana R. Homann, Stefan Burger |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Lysis Computer science business.industry 010401 analytical chemistry Microfluidics Extraction (chemistry) 01 natural sciences DNA extraction 0104 chemical sciences 03 medical and health sciences Cartridge Reagent Laboratory centrifuge User interface Process engineering business 030304 developmental biology |
| Zdroj: | 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). |
| Popis: | The LabSlice XL is a novel centrifugal microfluidic platform for the extraction of nucleic acids from pathogens in complex sample matrices such as process water. For the first time, a standard filter membrane used to collect pathogens from large volumes is directly inserted into a microfluidic cartridge. A standard laboratory centrifuge is applied to automatize the bio-chemical procedure of lysate purification, DNA extraction, and PCR preparation. Convenient user interfaces and complete reagent pre-storage ensure user friendliness. Experiments show that the microfluidic process flow works as intended for three actual process water samples. The automated processing yields approximately 43% of the amount of DNA compared to manual processing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|