Retention of B Cell Function in Organ-Cultured Rat Pancreatic Islet
| Autor: | Junko Ono, Takashi Ikegami, Michio Fukuma, Ryosaburo Takaki |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
endocrine system
medicine.medical_specialty geography geography.geographical_feature_category Physiology Pancreatic islets Endoplasmic reticulum Degranulation Radioimmunoassay Cell Biology General Medicine Biology Organ culture Islet In vitro Endocrinology medicine.anatomical_structure Internal medicine medicine Molecular Biology B cell |
| Zdroj: | Cell Structure and Function. 1:291-297 |
| ISSN: | 1347-3700 0386-7196 |
| DOI: | 10.1247/csf.1.291 |
| Popis: | Organ culture of the pancreatic islets of a 14-day old WKA rat was achieved on a millipore filter supported by a small petri dish filled with liquid medium. During the 9-day culture period, the architecture of the islets was preserved. Morphological alterations of B cells were found under electron microscopy after increasing the concentration of glucose. These changes in B cells included degranulation and formation of well-developed rough endoplasmic reticulum. A few mitotic figures were observed among B cells of islets cultured with glucose at 3 mg per ml for nine days. The morphological observations were supported by radioimmunoassay of insulin-release into the medium. Organ-cultured islets in 3 mg per ml of glucose released approximately nine times as much insulin into the medium during each three day period assayed as islets in 1 mg per ml of glucose. This culture system provides a useful tool for investigating the effects of various agents on the function of islet cells for prolonged periods in vitro. |
| Databáze: | OpenAIRE |
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