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This study was conducted to determine the iontransport mechanisms in the normal mouse cecum andcompare them to an inbred mouse model of colitis. TheUssing chamber-voltage clamp technique was used to monitor the short circuit current(Isc). The basal Isc in the normalcecum was 82.6 ± 5.8 μA/cm2. It wasnot affected by bumetanide, 9-anthracene carboxylate,amiloride, and phenamil or by removal of Cl- ions; but was abolished by theremoval of Na+ ions. Flux measurementsrevealed the presence of neutral NaCl transport. In thecolitic cecum, the basal current was significantlyhigher than the normal cecum. Basal current in the normal cecum wasdue primarily to Na+ absorption through aNa+ channel, while in the colitic cecum itwas due to Cl- ion secretion. cAMP additionin colitic cecum did not increase Cl- secretion, further suggestingthat the tissue is already secreting at a maximalrate. |
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