| Autor: |
Tomoko Hosokawa, Teruyuki Kanada, Rina Kato, Toshiko Suzuki-Yamamoto, Hiromi Yamashita, Miyuki Yokoro, Shioka Irino, Hideaki Tsuji, Takahiko Hada, Shiori Hirano, Yuki Kawakami, Shinobu Doi, Hirokazu Kobayashi, Chikao Yutani, Yoshitaka Takahashi, Akiko Yoshioka, Tomoko Morinaka, Hiroyuki Itabe, Masumi Kimoto |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Food Chemistry. 131:1069-1075 |
| ISSN: |
0308-8146 |
| DOI: |
10.1016/j.foodchem.2011.10.011 |
| Popis: |
Oxidative modification of low density lipoprotein (LDL) is one of the critical steps for the development of atherosclerosis and leucocyte-type 12-lipoxygenase, highly expressed in macrophages, has been suggested to play an essential role in this process. In the present study, we show that guava leaf extracts inhibited, not only the leucocyte-type 12-lipoxygenase activity, but also LDL oxidation, mediated by the enzyme-overexpressing macrophage-like J774A.1 cells. Oral administration of guava leaf extracts to apoE-knockout mice at 100 mg of dry extracts/kg of body weight, once a day for 16 weeks, significantly reduced the area of atherogenic lesions developed in the aorta and aortic sinus. The major components inhibiting leucocyte-type 12-lipoxygenase contained in guava leaf extracts were identified as ethyl gallate and quercetin. The inhibitory effects of guava leaf extracts on the leucocyte-type 12-lipoxygenase activity, as well as on cell-mediated LDL oxidation, might be involved in the antiatherogenic effect of the extracts. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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