| Autor: |
M. Darby, M. D. McCarthy, C. Wiens, S. Atherton, I. Oertel |
| Rok vydání: |
1960 |
| Předmět: |
|
| Zdroj: |
American Journal of Physiology-Legacy Content. 198:911-915 |
| ISSN: |
0002-9513 |
| DOI: |
10.1152/ajplegacy.1960.198.4.911 |
| Popis: |
Single subcutaneous injections of supernatant, the equivalent of that from one spleen obtained from either normal or 3-week postburn rat donors and homogenized in phosphate buffer, were given to normal recipient rats in three weight groups, 45–60 gm, 73–84 gm and 245–295 gm. Phosphate buffer controls were injected concomitantly. Effects on the recipients' hematocrits and circulating reticulocyte percentages were observed. Both ‘burned’ and ‘normal’ spleen supernatant depressed hematocrits and reticulocytes. The greatest effect was noted in the 73–84-gm weight class. In a second series of experiments, repeated injections were given for 5 days to normal animals weighing between 190 and 210 gm. The effects of supernatant from both ‘burned’ and ‘normal’ spleens on hematocrits and reticulocyte numbers were compared with those from similarly prepared supernatants of liver and kidney and sera injections. The tissue extracts were given on the basis of 1 gm of the initial wet weight of tissue or serum per 100 gm of body weight of recipient. Only the splenic injections resulted in a progressively sustained hematocrit and reticulocyte depression, with the greatest effect produced by the ‘burned’ spleen extract. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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