Low level epifluorescent detection of nanoparticles and DNA on dielectrophoretic microarrays
| Autor: | Michael J. Heller, Avery Sonnenberg, James Mccanna |
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| Rok vydání: | 2013 |
| Předmět: |
Analyte
Microscope Microarray Confocal General Engineering General Physics and Astronomy General Chemistry Dielectrophoresis Biology Molecular biology General Biochemistry Genetics and Molecular Biology law.invention chemistry.chemical_compound chemistry law Drug delivery General Materials Science DNA microarray DNA Biomedical engineering |
| Zdroj: | Journal of Biophotonics. 7:863-873 |
| ISSN: | 1864-063X |
| DOI: | 10.1002/jbio.201300046 |
| Popis: | Common epifluorescent microscopy lacks the sensitivity to detect low levels of analytes directly in clinical samples, such as drug delivery nanoparticles or disease related DNA biomarkers. Advanced systems such as confocal microscopes may improve detection, but several factors limit their applications. This study now demonstrates that combining an epifluorescent microscope with a dielectrophoretic (DEP) microelectrode array device enables the detection of nanoparticles and DNA biomarkers at clinically relevant levels. Using DEP microarray devices, nanoparticles and DNA biomarkers are rapidly isolated and concentrated onto specific microscopic locations where they are easily detected by epifluorescent microscopy. In this study, 40nm nanoparticles were detected down to 2–3 × 103/ul levels and DNA was detected down to the 200 pg/ml level. The synergy of epifluorescent microscopy and DEP microarray devices provides a new paradigm for DNA biomarker diagnostics and the monitoring of drug delivery nanoparticle concentrations. (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| Databáze: | OpenAIRE |
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