Photodynamic therapy: Optimal treatment parameters in murine fibrosarcoma

Autor: F. A. H. Al-Watban, G. King, R. Brannon, T. Altamero
Rok vydání: 1987
Předmět:
Zdroj: Lasers in Medical Science. 2:55-62
ISSN: 1435-604X
0268-8921
DOI: 10.1007/bf02594132
Popis: We have investigated the efficacy of photodynamic therapy (PDT) by using two preparations of haematoporphyrin derivative (HPD) that demonstrated different biological activities against experimental murine fibrosarcoma RIF-1 in C3H/He mice. We have been able to demonstrate a minimum clonogenic survival rate of 0.25% by using the more active HPD at 20 mg/kg with a 40-h retention time and a total laser light dose of 100 J/cm2. Further, we noted that clonogenic survival rates of 21.6% and 25.0% respectively could be achieved by using the less active HPD (at 20 mg/kg) with a laser light dose of 150 J/cm2, or the more active HPD (at 20 mg/kg) with a laser light dose of 20 J/cm2. In both cases necrosis of the surrounding normal tissue was absent. Necrosis of the normal tissue surrounding the tumour was shown to be associated with high laser energy (100 J/cm2 and higher) in conjunction with a high dose (20 mg/kg) of the more active HPD. A comparison of survival curves as a function of laser energy for the RIF-1 cells following PDT with the two different preparations of HPD showed a difference in the kinetics of cell death. A curve with a shoulder region and aDo (mean lethal dose) of 41 J/cm2 was obtained when the less active HPD was used, whereas PDT using the more active HPD resulted in a curve with no shoulder and aDo of 16 J/cm2.
Databáze: OpenAIRE
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