Application of high-performance anion-exchange chromatography with pulsed amperometric detection to sugar analysis in citrus juices

Autor: W. W. Widmer, D. R. White
Rok vydání: 1990
Předmět:
Zdroj: Journal of Agricultural and Food Chemistry. 38:1918-1921
ISSN: 1520-5118
0021-8561
DOI: 10.1021/jf00100a008
Popis: A high-performance anion-exchange chromatographic method that uses an alkaline mobile phase on a pellicular quaternary amine resin column at ambient temperature, coupled with pulsed amperometric detection, has been applied to the analysis of the three major sugars: glucose, fructose, and sucrose. Data were compared with those obtained from independent analysis by HPLC on an amino-bonded silica column with refractive index detection. Limits of detection were estimated to be 45 ng for glucose, 60 ng for fructose, and 300 ng for sucrose
Databáze: OpenAIRE