Cutting Edge: The Transcription Factor Sox2 Regulates AID Expression in Class-Switched B Cells
| Autor: | Wei-Feng Yen, Laura Nicolas, Zara N. Saldanha, Rahul Sharma, Lauren J. DiMenna, Jayanta Chaudhuri |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Genome instability Effector Immunology chemical and pharmacologic phenomena Chromosomal translocation Cytidine deaminase Biology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology SOX2 chemistry Immunoglobulin class switching Immunology and Allergy Transcription factor DNA |
| Zdroj: | The Journal of Immunology. 198:2244-2248 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.1502266 |
| Popis: | IgH class switch recombination (CSR) occurs through the deliberate introduction of activation-induced cytidine deaminase (AID)-instigated DNA double-strand breaks into the IgH loci. Because double-strand breaks are generally highly toxic, mechanisms that regulate AID expression are of much relevance to CSR and genomic integrity; however, effectors of such regulatory processes are still poorly understood. In this article, we show that the transcription factor sex determining region Y-box 2 (Sox2) is expressed in activated B cells, but almost exclusively in those that have undergone CSR. We demonstrate that enforced expression of Sox2 in splenic B cells severely inhibits AID expression and CSR, whereas deletion of Sox2 increases the frequency of IgH:c-Myc translocations. These results suggest that Sox2 may regulate AID expression in class-switched B cells to suppress genomic instability associated with CSR. |
| Databáze: | OpenAIRE |
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