Efficient biosynthesis of enantiopure tolvaptan by utilizing alcohol dehydrogenase-catalyzed enantioselective reduction
| Autor: | Honglei Zhang, Zhi-Dong Xu, Chao-Hong Pei, Chao Zhang, Wei Li, Mengnan Han |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
biology 010405 organic chemistry Bioconversion Enantioselective synthesis medicine.disease_cause Formate dehydrogenase 01 natural sciences Pollution Combinatorial chemistry 0104 chemical sciences chemistry.chemical_compound Enantiopure drug Biosynthesis chemistry 010608 biotechnology medicine biology.protein Environmental Chemistry Enantiomeric excess Escherichia coli Alcohol dehydrogenase |
| Zdroj: | Green Chemistry. 20:1224-1227 |
| ISSN: | 1463-9270 1463-9262 |
| DOI: | 10.1039/c7gc03679e |
| Popis: | Using whole cells of Escherichia coli co-expressing alcohol dehydrogenase (PsADH) and formate dehydrogenase (CpFDH) in a biphasic aqueous–soybean oil system is shown to be an efficient method for the biosynthesis of enantiopure tolvaptan. In this system, (S)-tolvaptan with an optical purity of 99.5% and a bioconversion efficiency of 86.1% was achieved. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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