Effects of substratum wettability and molecular topography on the initial adhesion of bacteria to chemically defined substrataab

Autor: K Mark Wiencek, Madilyn Fletcher
Rok vydání: 1997
Předmět:
Zdroj: Biofouling. 11:293-311
ISSN: 1029-2454
0892-7014
DOI: 10.1080/08927019709378338
Popis: Self‐assembled monolayers (SAMs) constructed from alkanethiols, whose surface composition and wettability can be rigorously characterized, were used to investigate bacterial adhesion to surfaces. Mixtures of methyl‐terminated and hydroxyl‐terminated alkanethiols were used to produce a series of SAMs with different wettabilities for water and hexadecane. Also, mixtures of 11‐carbon and 18‐carbon SAMs were used to determine the influence of molecular topography on bacterial adhesion and surface wettability. In particular, attachment to mixed chain‐length SAMs in which hydroxyl‐terminated, 11‐carbon alkanethiols were mixed with methyl‐terminated, 18‐carbon alkanethiols was evaluated, to determine whether masking of the polar hydroxyl groups by the longer alkane chains affected bacterial attachment to these surfaces. Substratum wettability was evaluated by measuring the contact angles of water and hexadecane. The kinetics of attachment of an estuarine pseudomonad (MI‐1A) to these surfaces in a laminar flow ch...
Databáze: OpenAIRE