Characterisation of FXTAS related isolated intranuclear protein inclusions using laser tweezers Raman spectroscopy
Autor: | Tobias J. Moritz, Denise M. Krol, Stephen M. Lane, Sebastian Wachsmann-Hogiu, James A. Brunberg, James W. Chan |
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Rok vydání: | 2009 |
Předmět: |
Chemistry
Analytical chemistry Dihedral angle medicine.disease Mass spectrometry symbols.namesake Nuclear magnetic resonance Optical tweezers Molecular vibration medicine symbols Side chain General Materials Science Tyrosine Raman spectroscopy Spectroscopy Fragile X-associated tremor/ataxia syndrome |
Zdroj: | Journal of Raman Spectroscopy. 41:33-39 |
ISSN: | 0377-0486 |
Popis: | We report the analysis of the vibrational modes of intranuclear protein inclusions isolated from the brain of human subjects with the Fragile X-associated tremor/ataxia syndrome (FXTAS). In this preliminary study, Raman spectra of optically trapped inclusions were measured and analysed to determine protein composition and structure. Our main findings are as follows: (1) The spectra of protein inclusions are characteristic of H2A and H2B histones, which correlate with previous mass spectrometry (MS) studies; (2) Tyrosine is present in its OH form and exposed at the protein surface; (3) Zn and to a lesser extent Cu bound to histidine side chains are detected in the inclusions; (4) The tryptophan side-chain torsion angle is calculated to be 102°; (5) Several potential spectroscopic markers for the inclusions of FXTAS are identified. These results show the capability of using laser tweezers Raman spectroscopy to identify protein inclusions in a non-perturbative way and to gain further insight into the pathogenesis and progression of FXTAS in human subjects and in experimental models of this disorder. Copyright © 2009 John Wiley & Sons, Ltd. |
Databáze: | OpenAIRE |
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