Discrimination among Japanese Species of the Orius Flower Bugs (Heteroptera: Anthocoridae) Based on PCR-RFLP of the Nuclear and Mitochondrial DNAs

Autor:	Takashi Noda, Masahiko Muraji, Toru Shimizu, Kenjiro Kawasaki
Rok vydání:	2004
Předmět:	Genetics Ecology biology Heteroptera Ribosomal RNA biology.organism_classification Anthocoridae Restriction enzyme Orius Animal Science and Zoology Internal transcribed spacer Restriction fragment length polymorphism Agronomy and Crop Science Biotechnology Tantillus
Zdroj:	Japan Agricultural Research Quarterly: JARQ. 38:91-95
ISSN:	2185-8896 0021-3551
DOI:	10.6090/jarq.38.91
Popis:	A PCR-RFLP-based method of species identification was considered for 5 Japanese species of Orius Wolff flower bugs, Orius strigicollis (Poppius), O. minutus (Linnaeus), O. sauteri (Poppius), O. nagaii Yasunaga, and O. tantillus (Motschulsky). Nucleotide sequences of the internal transcribed spacer 1 of the nuclear ribosomal gene and a portion of mitochondrial cytochrome oxidase subunit I gene were compared among species and recognition sites of diagnostically useful restriction enzymes were examined. The PCR-RFLP analysis using 108 individuals of 26 laboratory strains confirmed that the 5 species could be correctly identified by banding patterns generated using these DNA regions. Because our PCR primers can amplify DNA fragments from DNA template extracted from both freshly killed insects and dried specimens stored at room conditions, the PCR-RFLP-based method was considered useful for analyses of field populations in which researchers store and accumulate field-collected samples before they perform laboratory examinations.
Databáze:	OpenAIRE
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