Mechanical and Chemical Predifferentiation of Mesenchymal Stem Cells Into Cardiomyocytes and Their Effectiveness on Acute Myocardial Infarction
| Autor: | Mohammad Molazem, Saeed Farzad Mohajeri, Mohammad Ali Shokrgozar, Susan Amin, Hossein Gholami, Baharak Abd Emami, Mohammad Mehdi Dehghan, Nooshin Haghighipour, Elena Mahmoudi, Seyyed Hossein Marjanmehr |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cardiac function curve medicine.medical_specialty Ejection fraction business.industry Mesenchymal stem cell Biomedical Engineering Medicine (miscellaneous) Adipose tissue Bioengineering General Medicine 030204 cardiovascular system & hematology medicine.disease Biomaterials Cell therapy Transplantation 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Internal medicine Cardiology Medicine Autologous transplantation Myocardial infarction business |
| Zdroj: | Artificial Organs. 42:E114-E126 |
| ISSN: | 0160-564X |
| Popis: | Myocardial infarction is one of the leading causes of death all over the world. Mesenchymal stem cells (MSCs) transplantation has shown a promising potential to recovery of ischemic heart disease due to their capability in differentiating into cardiac cells. However, various investigations have been performed to optimize the efficacy of cardiac cell therapy in recent years. Here, we sought to interrogate the effect of autologous transplantation of undifferentiated and predifferentiated adipose and bone marrow-derived MSCs in a rabbit model of myocardial infarction and also to investigate whether cardiac function could be improved by mechanically induced MSCs via equiaxial cyclic strain. The two sources of MSCs were induced toward cardiomyocyte phenotype using mechanical loading and chemical factors and thereafter injected into the infarcted myocardium of 35 rabbits. Echocardiography and histopathology studies were used to evaluate cardiac function after 2 months. The results demonstrated significant scar size reduction and greater recovery of left ventricle ejection fraction after transplantation of predifferentiated cells, though the differences were not significant when comparing mechanically with chemically predifferentiated MSCs. Thus, although there was no significant improvement in infarcted myocardium between chemically and mechanically predifferentiated MSCs, mechanically induced cells are more preferred due to lack of any chemical intervention and cost reasonableness in their preparation method. Outcomes of this study may be useful for developing future therapeutic strategies, however long-term assessments are still required to further examine their effectiveness. |
| Databáze: | OpenAIRE |
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