Early fragment removal on in vitro fertilization day 2 significantly improves the subsequent development and clinical outcomes of fragmented human embryos
| Autor: | Hong-Gil Sun, Ji-Young Park, Hee-Jun Chi, Il-Hae Park, Chang-Seok Yoo, Youn-Young Kim, Seok-Gi Kim, Jae-Won Kim, Kyeong-Ho Lee, Humdai Park, Su-Jin Kwak |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
030219 obstetrics & reproductive medicine In vitro fertilisation medicine.diagnostic_test Hatching Fragment (computer graphics) medicine.medical_treatment Clinical pregnancy Embryo Blastomere Biology Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Reproductive Medicine embryonic structures Biopsy medicine Fragmentation (cell biology) |
| Zdroj: | Clinical and Experimental Reproductive Medicine. 45:122-128 |
| ISSN: | 2233-8241 2233-8233 |
| DOI: | 10.5653/cerm.2018.45.3.122 |
| Popis: | Objective To determine whether fragment removal on in vitro fertilization (IVF) day 2 improved the subsequent development and pregnancy outcomes of fragmented embryos compared to similar-grade embryos without fragment removal. Methods This study was a retrospective analysis involving 191 IVF cycles in which all embryos had over 10% fragmentation (grade 3 or 4) on day 2 of the IVF-embryo transfer cycle from March 2015 to December 2017. IVF cycles were divided into the fragment removal group (n=87) and the no fragment removal group (n=104) as a control cohort. Before fragment removal, embryos with fragmentation on day 2 were incubated in Ca2+- and Mg2+-free biopsy medium under paraffin oil for 30 minutes. Microsurgical fragment removal was performed with later-assisted hatching and a handmade suction micropipette that had an outer diameter of 30 µm. Results There were no significant differences in the characteristics of the patients between the control and the fragment removal groups. After fragment removal and subsequent in vitro culture for 24 hours, the number of blastomeres (7.1±1.7 vs. 6.9±1.6) was comparable between the transferred embryos in the two groups, but the morphological grade of the embryos in the fragment removal group (1.9±0.7) was significantly higher than that of the control group (3.1±0.5, p |
| Databáze: | OpenAIRE |
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