Expression and localization of recombinant human B2 receptors in the methylotrophic yeast Pichia pastoris

Autor:	Haoxing Zhang, Guangxiao Yang, Daleng Shen, Tianlei Liu, C. Q. Wu
Rok vydání:	2006
Předmět:	Expression vector medicine.diagnostic_test Biology biology.organism_classification Molecular biology Yeast Alcohol oxidase Pichia pastoris Green fluorescent protein law.invention Biochemistry Western blot law Genetics medicine Recombinant DNA Receptor
Zdroj:	Russian Journal of Genetics. 42:728-731
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795406070040
Popis:	The human bradykinin B2 receptor (B2R) fused with green fluorescent protein (GFP) at the C-terminal has been expressed in the methylotrophic yeast Pichia pastoris. In the expression vector, B2R gene was driven under the highly inducible promoter of alcohol oxidase 1 gene of P. pastoris. By fluorescence activated cell sorting (FACS) analysis and Western blot analysis, it was proved that B2R recombinant receptor proteins were expressed at a high level in the yeast. Furthermore, the transformants of P. pastoris were monitored with confocal microscopy, a strong green fluorescence was checked out. The recombinant B2R receptor proteins were mainly located on the plasma membrane proved by immunofluorescence microscopy.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::58933890daf05a69e5c494c0a2a14f1e https://doi.org/10.1134/s1022795406070040 Zobrazit plný text záznamu Full text from SpringerLink