Development and Validation of an RP-HPLC Method for Determination of Solasodine, A Steroidal Alkaloid
| Autor: | Mostafa Pournamdari, Shahram Kalantari Khandani, Mitra Mehrabani, Fariba Sharififar, Mahboobeh pakravanan, Abbas Pardakhty |
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| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | Journal of Young Pharmacists. 11:21-25 |
| ISSN: | 0975-1505 0975-1483 |
| DOI: | 10.5530/jyp.2019.11.5 |
| Popis: | Objective: The aim of the present study was to optimize an RP-HPLC method for determination of solasodine, a steroidal alkaloid with various pharmacologic properties, in herbal medicinal preparations. Methods: Various chromatographic parameters including the composition of mobile phase and its organic and pH modifiers, mode of elution and column temperature were investigated using analytical C 18 columns as stationary phase. Acetonitrile and methanol were examined in combination with deionized water and different aqueous buffers as mobile phases. Results: A mixture of methanol:KH 2 PO 4 buffer (pH 2.5) at the ratio of 75:25 %v/v in an isocratic elution mode produced more symmetric, sharper peaks with better retention times and capacity factor than that of acetonitrile:KH 2 PO 4 buffer. The optimum column temperature was found at 25°C. The standard calibration curve showed a very good linearity (R 2 =0.9966) in the range of 1-25 μg/ml with acceptable inter-day and intra-day precisions and accuracy. In addition, the LOD and the LOQ of the method were determined at 0.2 and 0.7 μg/mL, respectively. Conclusion: This developed and validated method is simple and quite rapid and can be used for routine determination of solasodine in samples such as traditional medicinal preparations. Key words: Solasodine, Steroidal alkaloid, RP-HPLC, Validation. |
| Databáze: | OpenAIRE |
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