Oxidative phosphorylation in a thermophilic, facultative chemoautotroph,Hydrogenophilus thermoluteolus, living prevalently in geothermal niches
| Autor: | Kaori Watanabe, Satoshi Wakai, Yoshihiro Sambongi, Saori Ueshima, Hirofumi Nishihara, Naho Yamaguchi, Misa Masanari, Takumi Ikeda |
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| Rok vydání: | 2012 |
| Předmět: |
biology
ATP synthase Bioenergetics ATPase Thermophile Oxidative phosphorylation biology.organism_classification medicine.disease_cause Agricultural and Biological Sciences (miscellaneous) Biochemistry biology.protein medicine Electrochemical gradient Escherichia coli Ecology Evolution Behavior and Systematics Bacteria |
| Zdroj: | Environmental Microbiology Reports. 5:235-242 |
| ISSN: | 1758-2229 |
| DOI: | 10.1111/1758-2229.12005 |
| Popis: | Summary Hydrogenophilus is a thermophilic, facultative chemoautotroph, which lives prevalently in high temperature geothermal niches. Despite the environmental distribution, little is known about its oxidative phosphorylation. Here, we show that inverted membrane vesicles derived from Hydrogenophilus thermoluteolus cells autotrophically cultivated with H2 formed a proton gradient on the addition of succinate, dl-lactate, and NADH, and exhibited oxidation activity toward these three organic compounds. These indicate the capability of mixotrophic growth of this bacterium. Biochemical analysis demonstrated that the same vesicles contained an F-type ATP synthase. The F1 sector of the ATP synthase purified from H. thermoluteolus membranes exhibited optimal ATPase activity at 65°C. Transformed Escherichia coli membranes expressing H. thermoluteolus F-type ATP synthase exhibited the same temperature optimum for the ATPase. These findings shed light on H. thermoluteolus oxidative phosphorylation from the aspects of membrane bioenergetics and ATPase biochemistry, which must be fundamental and advantageous in the biogeochemical cycles occurred in the high temperature geothermal niches. |
| Databáze: | OpenAIRE |
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