Differential regulations of ASICs and TRPV1 by zinc in rat bronchopulmonary sensory neurons (712.3)

Autor:	Charles R. Moss, Qihai Gu, Zhanna V. Vysotskaya
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Antagonist TRPV1 chemistry.chemical_element Long-term potentiation Zinc Biochemistry Divalent Transient receptor potential channel chemistry.chemical_compound chemistry Genetics Biophysics Capsazepine Molecular Biology Ion channel Biotechnology
Zdroj:	The FASEB Journal. 28
ISSN:	1530-6860 0892-6638
DOI:	10.1096/fasebj.28.1_supplement.712.3
Popis:	Zinc, a divalent heavy metal ion and an essential trace element in body, functions as a signaling molecule that regulates a variety of neuronal functions. In this study, we examined the effect of zinc on two populations of acid sensitive ion channels, acid-sensing ion channels (ASICs) and transient receptor potential vanilloid receptor-1 (TRPV1), in rat bronchopulmonary sensory neurons. In our patch-clamp recordings, ASIC currents were induced by a pH drop from 7.4 to 6.8 or 6.5 in the presence of capsazepine (10 µM), a specific TRPV1 antagonist. Our data showed that pretreatment with zinc (50 or 300 µM, 2 min) displayed different effects on the two distinct phenotypes of ASIC currents: a marked potentiation on ASIC channels with fast kinetics of activation and deactivation, or no significant effect on ASIC currents with slow activation and deactivation. On the other hand, pretreatment with zinc significantly inhibited the acid (pH 5.3 or 5.5)-induced TRPV1 currents in these sensory neurons. The inhibitio...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::587b540cb32d3f9f6bbaef8d8e655992 https://doi.org/10.1096/fasebj.28.1_supplement.712.3 Zobrazit plný text záznamu Plný text