Localization of a DNA replication origin and termination zone on chromosome III of Saccharomyces cerevisiae

Autor:	J Zhu, C S Newlon, J A Huberman
Rok vydání:	1992
Předmět:	Cell Biology Molecular Biology
Zdroj:	Molecular and Cellular Biology. 12:4733-4741
ISSN:	1098-5549 0270-7306
Popis:	Two-dimensional gel electrophoretic replicon mapping techniques were used to identify all functional DNA replication origins and termini in a 26.5-kbp stretch in the left arm of yeast chromosome III. Only one origin was detected; it coincided with an ARS element (ARS306), as have all previously mapped yeast origins. A replication termination region was identified in a 4.3-kbp stretch at the telomere-proximal end of the investigated region, between the origin identified in this paper and the neighboring, previously mapped, ARS305-associated origin (previously called the A6C origin). Termination does not occur at a specific site; instead, it appears to be the consequence of replication forks converging in a stretch of DNA of at least 4.3 kbp.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::58675a57ee22f11884da2b32cbda9f95 https://doi.org/10.1128/mcb.12.10.4733 Zobrazit plný text záznamu