Sequence Variations Among 17 New Radish Isolates of Turnip mosaic virus Showing Differential Pathogenicity and Infectivity in Nicotiana benthamiana, Brassica rapa, and Raphanus sativus
| Autor: | Young Pyo Lim, Junsu Gong, Ik-Hyun Kim, Hyekyoung Ju, Hyoun-Sub Lim, Su Ryun Choi, Wen-Xing Hu, Jungkyu Kim, John Hammond, Eun-Young Seo, In-Sook Cho, Jae-Yeong Han |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Infectivity biology Phylogenetic tree food and beverages Raphanus Nicotiana benthamiana Plant Science Plant disease resistance biology.organism_classification 01 natural sciences Virology 03 medical and health sciences 030104 developmental biology Plant virus Brassica rapa Turnip mosaic virus Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | Phytopathology®. 109:904-912 |
| ISSN: | 1943-7684 0031-949X |
| Popis: | Infectious clones were generated from 17 new Korean radish isolates of Turnip mosaic virus (TuMV). Phylogenetic analysis indicated that all new isolates, and three previously characterized Korean radish isolates, belong to the basal-BR group (indicating that the pathotype can infect both Brassica and Raphanus spp.). Pairwise analysis revealed genomic nucleotide and polyprotein amino acid identities of >87.9 and >95.7%, respectively. Five clones (HJY1, HJY2, KIH2, BE, and prior isolate R007) had lower sequence identities than other isolates and produced mild symptoms in Nicotiana benthamiana. These isolates formed three distinct sequence classes (HJY1/HJY2/R007, KIH2, and BE), and several differential amino acid residues (in P1, P3, 6K2, and VPg) were present only in mild isolates HJY1, HJY2, and R007. The remaining isolates all induced systemic necrosis in N. benthamiana. Four mild isolates formed a phylogenetic subclade separate from another subclade including all of the necrosis-inducing isolates plus mild isolate KIH2. Symptom severity in radish and Chinese cabbage genotypes was not correlated with pathogenicity in N. benthamiana; indeed, Chinese cabbage cultivar Norang was not infected by any isolate, whereas Chinese cabbage cultivar Chusarang was uniformly susceptible. Four isolates were unable to infect radish cultivar Iljin, but no specific amino acid residues were correlated with avirulence. These results may lead to the identification of new resistance genes against TuMV. |
| Databáze: | OpenAIRE |
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