Preparation of a Branched DNA Self-Assembled Monolayer toward Sensitive DNA Biosensors

Autor:	Yosuke Sakao, Nobuo Ueno, Masahiko Hara, Fumio Nakamura, Isaiah N. Gatuna, Eisuke Ito, and Fumio S. Ohuchi
Rok vydání:	2003
Předmět:	Oligonucleotide Chemistry Mechanical Engineering Substrate (chemistry) Bioengineering Nanotechnology Self-assembled monolayer General Chemistry Condensed Matter Physics chemistry.chemical_compound Monolayer Biophysics General Materials Science A-DNA Surface plasmon resonance Biosensor DNA
Zdroj:	Nano Letters. 3:1083-1086
ISSN:	1530-6992 1530-6984
Popis:	To realize an efficient hybridization of oligonucleotides, a DNA self-assembled monolayer (SAM) composed of branched probe DNA containing single- and double-stranded portions was prepared on a gold substrate. Hybridization using the SAM was monitored in situ by surface plasmon resonance (SPR). By controlling the surface coverage of the probe DNA, hybridization efficiency was optimized. SPR results strongly indicate that all probe sites are accessible for hybridization under optimized conditions regardless of the position.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::585a504cd007c20f4eea565dccb21bec https://doi.org/10.1021/nl0342794 Zobrazit plný text záznamu