Identification of flavonoids in Hymenaea martiana Hayne (Fabaceae) by HPLC-DAD-MSn analysis
| Autor: | Larissa Araújo Rolim, Alessandra Gomes Marques Pacheco, Norberto Peporine Lopes, Alexsandro Branco, Alan Diego da Conceição Santos, Gibson Gomes de Oliveira, Celso A. Câmara, Tânia Maria Sarmento da Silva, Jacqueline Nakau Mendonça, Lívia Macedo Dutra, Telma Maria Guedes da Silva, Ana Paula de Oliveira, Jackson Roberto Guedes da Silva Almeida |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
biology 010405 organic chemistry Organic Chemistry Ethyl acetate Engeletin Fraction (chemistry) Plant Science Fabaceae biology.organism_classification 01 natural sciences Biochemistry Quercitrin 0104 chemical sciences Analytical Chemistry 010404 medicinal & biomolecular chemistry chemistry.chemical_compound chemistry Taxifolin Hymenaea Astilbin |
| Zdroj: | Natural Product Research. 35:2414-2419 |
| ISSN: | 1478-6427 1478-6419 |
| Popis: | Hymenaea martiana is a native tree known in Brazil as 'jatoba' and used in folk medicine to treat pain and inflammation. The aim of this work was to identify the flavonoids present in the crude ethanolic extract and ethyl acetate fraction using HPLC-DAD and LC-MSn analysis. The ethanolic extract was partitioned to obtain the ethyl acetate fraction. The analysis of astilbin content also was carried out by HPLC analysis. HPLC-DAD-ESI/MSn analysis of the ethanolic extract and ethyl acetate fraction revealed the presence of eleven peaks in the chromatograms, and all these peaks were identified: taxifolin, eucryphin, astilbin and 3 diastereoisomers, engeletin and 2 diastereoisomers, quercitrin and 2,6,3',4'-tetrahydroxy-2-benzylcoumaran-3-one. The ethyl acetate fraction had a higher astilbin concentration (151.87 µg/mL) than the ethanolic extract (40.13 µg/mL). In conclusion, the species could be considered a good source of flavonoids, which can be related to the main chemotaxonomic markers for the genus Hymenaea. |
| Databáze: | OpenAIRE |
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