Molecular Identification of Banana Bunchy Top Virus Associated with Banana Plantation in Uttar Pradesh
| Autor: | Priyanka Pal, Chhavi Malik, Dharmendra Pratap, Deepak Panwar |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | International Journal of Current Microbiology and Applied Sciences. 11:145-152 |
| ISSN: | 2319-7706 2319-7692 |
| Popis: | Banana is the most important fruit in India after mango .Its cultivation is affected by various diseases. Among them BBTD (Banana bunchy top disease) caused by BBTV (Banana bunchy top virus) is one of the most important constraints in banana production worldwide. BBTV is a complex circular single stranded DNA virus with multiple genomic components (DNA1-6) and causes heavy economic loss in banana cultivated areas. BBTV is transmitted by its vector (Pentalonia nigronervosa). On the basis of symptoms such as green streaks on pseudostem, stunted growth, leaf atrophy, bunchy top appeared on infected plants, leaf samples were taken for isolating genomic DNA using CTAB method with some modification in Selvarajan protocol. DNA was quantified (50ug/lit DNA). PCR amplification was performed on all isolates from jail chungi, Skin center, CCSU Campus, Healthy sample Meerut, field sample no.1 (District Lakhimpur khedi) U.P for early detection of virus. PCR done with the help of specific primers CP(Coat protein gene) and PR(Partial Replicase gene) using total extracted DNA.The Agarose gel was loaded with ladder DNA (1kb), followed by sample DNA. Among DNA samples, all showed amplification by specific primer pair of BBTV.During the study it has been clear that the isolates of U.P were infected by BBTV. So, based on this analysis, it can be concluded that, detection of virus early and proper eradication of infectious plants is very important before passing of virus to another healthy plants. |
| Databáze: | OpenAIRE |
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