On-line and off-line monitoring of the production of alkaline serine protease by Bacillus licheniformis
| Autor: | F. Spitzenberger, Karl Schügerl, Bernd Hitzmann, Gerlinde Kretzmer, A.B. Van Putten |
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| Rok vydání: | 1995 |
| Předmět: |
Serine protease
Chromatography Protease biology medicine.medical_treatment Subtilisin Maltose biology.organism_classification Phosphate Biochemistry Analytical Chemistry chemistry.chemical_compound chemistry biology.protein Urea medicine Environmental Chemistry Bacillus licheniformis Amylase Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 317:247-258 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/0003-2670(95)00420-3 |
| Popis: | Subtilisin Carlsberg alkaline serine protease was produced by Bacillus licheniformis on complex medium in a stirred tank reactor and the turbidity and culture fluorescence were monitored in situ, absorbance on-line and off-line, (dry) sediment, (dry) cell mass and DNA concentrations off-line. These data were correlated with each other. The cultivation medium composition: starch, maltose, glucose, urea, ammonia, phosphate concentrations and protease activity were monitored by a six-channel flow injection analysis system on-line and off-line. Protein concentration, α-amylase activity and intracellularly phosphate concentration were evaluated off-line. The on-line and off-line data agreed well. Differences in the on-line and off-line monitored protease activities are caused by the membrane fouling of the sampling system. |
| Databáze: | OpenAIRE |
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