Exploitation of Subtilisin BPN‘ as Catalyst for the Synthesis of Peptides Containing Noncoded Amino Acids, Peptide Mimetics and Peptide Conjugates

Autor:	Katsuhiro Kawashiro, Pamela Sears, Wilna J. Moree, Chi-Huey Wong, and Krista Witte
Rok vydání:	1997
Předmět:	chemistry.chemical_classification Serine protease biology Chemistry Stereochemistry Active site Peptide General Chemistry Biochemistry Catalysis Amino acid Colloid and Surface Chemistry Enzyme S-tag biology.protein Peptide bond Conjugate
Zdroj:	Journal of the American Chemical Society. 119:3942-3947
ISSN:	1520-5126 0002-7863
DOI:	10.1021/ja964399w
Popis:	The ability of the serine protease subtilisin BPN‘ to catalyze peptide bond formation between fragments containing noncoded amino acids, peptide mimetics, and peptide conjugates in a kinetic approach was explored. It was found that the enzyme accepts numerous of these types of compounds both as acyl donor and acyl acceptor. The results together with specificity studies reported by others provide an active site model as a guideline in the design of enzymatic synthesis of biologically important compounds.
Databáze:	OpenAIRE
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