Are VIDAS® anti-HEV IgM and IgG assays fit for reliable diagnosis of hepatitis E virus infections? Comparison & case story telling
| Autor: | Elizaveta Padalko, Lien Cattoir, Koen Vercauteren, An Boel, Hans De Beenhouwer, Kristien Van Vaerenbergh |
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| Rok vydání: | 2019 |
| Předmět: |
Natural course
business.industry viruses virus diseases Acute infection Anti hev igm General Medicine Comparison case medicine.disease_cause Story telling Virology digestive system diseases Serology 03 medical and health sciences 0302 clinical medicine Hepatitis E virus 030220 oncology & carcinogenesis Genotype medicine 030212 general & internal medicine business |
| Zdroj: | Acta Clinica Belgica. 76:25-31 |
| ISSN: | 2295-3337 1784-3286 |
| DOI: | 10.1080/17843286.2019.1655245 |
| Popis: | Objectives: Hepatitis E virus (HEV) genotype 3 is an emerging pathogen in developed countries. We evaluated the performance of two new serological assays for the detection of HEV, VIDAS® anti-HEV IgM and IgG. Methods: VIDAS® assays were performed on 77 clinical samples: 68 samples from patients suspected for HEV infection and 9 samples which previously tested positive for HEV IgM, IgG or HEV PCR. All samples were also tested using Wantai HEV assays. Cross-reactivity was assessed. To get a better view on the natural course of HEV infections, three clinical cases are described. Results: The concordance rate between VIDAS® and Wantai assays was good for HEV IgM (0.75,CI 0.52-0.98) and very good for HEV IgG (0.85,CI 0.72-0.98). Four samples tested borderline/positive with Wantai IgM but negative with VIDAS® IgM. All of these samples were HEV RNA negative, HEV IgG was positive in 2/4 samples. Five samples produced conflicting HEV IgG results. These tested positive with VIDAS® but negative with Wantai IgG. All five samples were HEV IgM and RNA negative. We detected no cross-reactivity. The clinical cases illustrate that HEV serology can still be negative in the very beginning of an acute infection. Conclusions: There is a good agreement between VIDAS® and Wantai anti-HEV IgM and IgG assays. Discrepant HEV IgM results probably reflect false positive Wantai IgM results (RNA-/IgG- samples) and longer-lasting positive Wantai IgM (RNA-/IgG+ samples). Discrepant HEV IgG results, could either represent resolved HEV infections (false negative Wantai IgG results) or false positive VIDAS® HEV IgG results. |
| Databáze: | OpenAIRE |
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