Proteomic Analysis of Pancreatic Secretory Trypsin Inhibitor/Tumor-Associated Trypsin Inhibitor from Urine of Patients with Pancreatitis or Prostate Cancer
| Autor: | Ulf-Håkan Stenman, Suvi Ravela, Leena Valmu |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences biology Chemistry Cancer Peptide medicine.disease Molecular biology 3. Good health 03 medical and health sciences Prostate cancer 0302 clinical medicine PstI 030220 oncology & carcinogenesis medicine biology.protein Biomarker (medicine) Pancreatitis Pancreatic Secretory Trypsin Inhibitor 030304 developmental biology Tumor marker |
| Zdroj: | Methods in Molecular Biology ISBN: 9781607617105 |
| Popis: | The development of proteomic methods, especially mass spectrometry, has brought new possibilities to tumor marker research. Pancreatic secretory trypsin inhibitor (PSTI), a common known biomarker for various malignancies, occurs on genetic variants that we are able to detect at the protein level with proteomic techniques using immunoaffinity capture prior to liquid chromatography-mass spectrometry (LC-MS). We also show that PSTI can be detected in urine from cancer patients using a two-step peptide enrichment technique and LC-MS. These results show that tumor-associated peptides can be detected in urine by proteomic techniques. |
| Databáze: | OpenAIRE |
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