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Publisher Summary This chapter discusses early development of nucleoside markers for cancer. In the early 197Os, the decision was made to initiate a program within the National Cancer Institute (NCI) in the development and evaluation of biochemical materials as potential biological markers to aid in the clinical management and assessment of cancer patients during the course of their illness. In early and mid-l970s, two developments led to major advances in the analysis of modified constituents of nucleic acids in physiological fluids. At the University of Missouri, research was pursued that focused both on the reversed-phase liquid chromatographic separation of the nucleosides and on the development of boronate gel affinity chromatography for isolation of the nucleosides from urine. The hydrolysis parameters were systematically investigated and the optimized hydrolysis conditions were established for quantitative hydrolysis of tRNAs. The relationships of parameters that were established as a result of these studies, allowed prediction of separation and increased the number of nucleosides that could be resolved in a single analysis to some 18 nucleosides, a number which would later more than double. |
