Identification of Molecular Markers that Segregate in a Simple Mendelian Fashion in a Controlled Cross of Asimina triloba
| Autor: | Tera M. Bonney, Kirk W. Pomper, Snake C. Jones, Shawn P. Brown, Robert L. Geneve |
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| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | HortScience. 35:551E-552 |
| ISSN: | 2327-9834 0018-5345 |
| DOI: | 10.21273/hortsci.35.4.551e |
| Popis: | The pawpaw [Asimina triloba (L.) Dunal] is a native plant found mainly in the southeastern and eastern United States, and its fruit has great potential as a new high-value crop in these regions. Although there are ≈45 named pawpaw cultivars, breeding for improvement of specific traits, such as fruit size and quality, is desirable. Our long-term goal is to utilize molecular marker systems to identify markers that can be used for germplasm diversity analyses and for the construction of a molecular genetic map, where markers are correlated with desirable pawpaw traits. The objective of this study was to identify random amplified polymorphic DNA (RAPD) markers that segregate in a simple Mendelian fashion in a controlled A. triloba cross. DNA was extracted from young leaves collected from field-planted parents and 20 progeny of the cross 1-7 × 2-54. The DNA extraction method used gave acceptable yields of ≈7 μg·g-1 of leaf tissue. Additionally, sample 260/280 ratios were ≈1.4, which indicated that the DNA was of high enough purity to be subjected to the RAPD methodology. Screening of 10-base oligonucleotide RAPD primers with template DNA from the parents and progeny of the cross has begun. We have identified two markers using Operon primer B-07 at 1.1 and 0.9 kb that segregate in a simple Mendelian fashion in progeny of the 1-7 × 2-54 cross. Other primers and controlled crosses will also be screened. |
| Databáze: | OpenAIRE |
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