Baboon corpus luteum: the effect of melatonin on in vitro progesterone production
| Autor: | Firyal S. Khan-Dawood, M. Yusoff Dawood |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Estrous cycle
endocrine system medicine.medical_specialty biology media_common.quotation_subject Obstetrics and Gynecology Ovary Luteal phase Human chorionic gonadotropin Melatonin medicine.anatomical_structure Endocrinology Reproductive Medicine biology.animal Internal medicine medicine Corpus luteum reproductive and urinary physiology hormones hormone substitutes and hormone antagonists Menstrual cycle Baboon medicine.drug media_common |
| Zdroj: | Fertility and Sterility. 59:896-900 |
| ISSN: | 0015-0282 |
| Popis: | Objective To determine the effect of melatonin on baboon corpus luteum (CL) cell progesterone (P) production. Design Five baboon CL obtained during the midluteal phase by luteectomy were dissociated using collagenase, and incubations were performed (50,000 cells per plate) in quadruplicate for 3hours at 37°C with melatonin (0.001 to 1.0ng/mL) (basal) or with melatonin and 10IU of human chorionic gonadotropin (hCG) (hCG-stimulated). Total P was measured by a specific radioimmunoassay. Main Outcome Measures Progesterone concentrations measured in the presence and absence of melatonin and hCG. Results Melatonin (0.01 to 1.0ng/mL) inhibited basal P production in all the CL (41.8±9.9ng P without melatonin compared with 32.2±2.0ng P, 28.4±2.1ng with 0.01 and 1.0ng/mL melatonin, respectively). Human chorionic gonadotropin-stimulated P production was significantly inhibited with as little as 0.01ng of melatonin (150.8±11.4ng with 10IU hCG versus 120.3±6.4ng with 10IU hCG and 1.0ng melatonin). The degree of inhibition in the hCG-stimulated cells was greater than in the nonstimulated cells. Melatonin at a concentration of 0.001ng/mL did not affect P production in both stimulated and nonstimulated cells. Serotonin in similar concentrations had no effect on luteal cell P production. Conclusion These findings indicate that melatonin exerts a suppressive effect on baboon dispersed luteal cell P production and thus may play a role in luteal function. |
| Databáze: | OpenAIRE |
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