Detection and cytospectrophotometric evaluation of enzyme activity in thyroid sections

Autor:	L. I. Pliner, V. Z. Klechikov
Rok vydání:	1974
Předmět:	chemistry.chemical_classification biology Thyroid Acid phosphatase General Medicine Optical density General Biochemistry Genetics and Molecular Biology Enzyme assay Staining Incubation period Enzyme medicine.anatomical_structure chemistry Biochemistry biology.protein medicine Peroxidase
Zdroj:	Bulletin of Experimental Biology and Medicine. 78:951-954
ISSN:	1573-8221 0007-4888
DOI:	10.1007/bf00803939
Popis:	The relationship between the optical density of thyroid sections from rats and guinea pigs, their thickness, and the incubation time, was investigated after staining for the enzymes lactate-, succinate-, NAD·H2-, and NADP·H2-tetrazolium reductases, acid phosphatase, and peroxidase. Analysis of the distribution curves of the optical density values showed that the requirements of photometry are best satisfied by sections 10μ thick with a density of 0.2–0.6 unit. The conditions under which the quantity of dye in thyroid sections increases as a linear function are described.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::5817d543de5d5efe57b725784a9bf387 https://doi.org/10.1007/bf00803939 Zobrazit plný text záznamu Full text from SpringerLink