| Autor: |
Peter R. Brayton, Leroy Hood, Elizabeth McLaughlin-Taylor, Jeffrey A. Frelinger, Richard C. Harmon, Anders Örn, Jerold G. Woodward, Robert S. Goodenow |
| Rok vydání: |
1982 |
| Předmět: |
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| DOI: |
10.1016/b978-0-12-722380-3.50032-5 |
| Popis: |
Mouse L cells ( H-2 k ) transfected with a genomic clone containing the gene for transplantation antigen H-2L express the H-2L d molecule on the cell surface in a form indistinguishable from the native molecules. To determine the applicability of this system to the study of major histocompatibility complex (MHC) gene function, several biological properties of a cloned H-2L d transformant, 8-5, were evaluated. When 8-5 cells were used as an immunogen in C3H ( H-2 k ) mice, an antiserum was produced with activity predominantly directed against H-2L d specificities. This illustrates the utility of this approach for defining previously uncharacterized class I gene products expressed in transformed cells. The H-2L d molecules expressed on 8-5 cells also function as direct target antigens and restricting elements for H-2L d specific and lymphocytic choriomeningitis virus specific cytotoxic T lymphocytes respectively. In both instances, recognition of 8-5 cells by effector T cells was blocked by addition of H-2L d specific antisera or monoclonal antibodies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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