A blind study of the polymerase chain reaction for the detection of mycobacterium tuberculosis DNA
| Autor: | A. Bourgoin, V. Lalande, E. Carpentier, M. Dailloux, F. Doucet-Populaire, B. Carbonnelle, C. Bollet, J. Grosset, J. Texier-Maugein, A. Vachée, D. Moinard |
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| Rok vydání: | 1996 |
| Předmět: |
Pulmonary and Respiratory Medicine
Tuberculosis biology Immunology medicine.disease biology.organism_classification Microbiology Virology law.invention Mycobacterium tuberculosis chemistry.chemical_compound Blind study chemistry Multicenter study law medicine Mycobacterium tuberculosis DNA Insertion sequence DNA Polymerase chain reaction |
| Zdroj: | Tubercle and Lung Disease. 77:358-362 |
| ISSN: | 0962-8479 |
| DOI: | 10.1016/s0962-8479(96)90102-1 |
| Popis: | Setting: Nine French laboratories routinely involved in mycobacterial work. Objective: To assess the detection of Mycobacterium tuberculosis in experimental samples by polymerase chain reaction (PCR) using the insertion sequence IS 6110 as a target for deoxyribonucleic acid (DNA) amplification. Design: Nine laboratories participated in a blind study of the detection of M. tuberculosis by PCR in 20 coded samples containing either a definite number of M. tuberculosis complex (positive samples) or environmental mycobacteria (four samples) or no mycobacteria (five samples). Results: Five laboratories reported false-positive PCR results, with an average rate of 7%. All laboratories except one reported positive PCR results for samples containing 10 5 cfu/ml or more. M. tuberculosis DNA was detected in two thirds of samples containing 10 4 and 10 3 cfu/ml, and in one third of the samples containing 10 2 cfu/ml. Conclusion: The results of the study suggest that PCR using IS 6110 as a target for DNA amplication is neither very sensitive nor really specific for the detection of M. tuberculosis . |
| Databáze: | OpenAIRE |
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