Identification of Differentially Expressed Putative Nitrate Transporter Genes in Lettuce
| Autor: | Ghassen Abid, H. Chikh-Rouhou, Nesrine Razgallah, Mahmoud M’hamdi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology food and beverages Lactuca Transporter Plant Science biology.organism_classification 01 natural sciences law.invention 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Nitrate chemistry Nitrate transport law Gene expression Botany Cultivar Agronomy and Crop Science Gene Polymerase chain reaction 010606 plant biology & botany |
| Zdroj: | International Journal of Vegetable Science. 23:390-399 |
| ISSN: | 1931-5279 1931-5260 |
| DOI: | 10.1080/19315260.2017.1309614 |
| Popis: | Nitrogen is important to plant growth and development, with nitrate being the major form absorbed by crops. Lettuce (Lactuca sativa L.) accumulates high levels of nitrate, which can cause harmful health effects in humans. Information is needed regarding accumulation of nitrate among lettuce genotypes. The lettuce cvs. Vitalia (type Butterhead); Great Lakes (type Iceberg); L01 (type Iceberg), and L02 (type Romaine) were used to determine expression patterns of nitrate transporter gene LsNRT in the cultivars and in nitrate accumulation. A computer modeling analysis indicated that genes LsNRT1 and LsNRT2 encode for low- and high-affinity nitrate transport, respectively, in lettuce. To better characterize nitrate transport in lettuce, the relative expression of these genes encoding nitrate transport was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). Higher nitrate accumulator cultivars posses higher nitrate transporter transcripts than low accumulators, indicating stronger ... |
| Databáze: | OpenAIRE |
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