Detection and Identification of Ligands for Mammalian RPTP Extracellular Domains
| Autor: | Andrew W. Stoker |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
animal structures biology Ligand Chemistry Cell adhesion molecule Phosphatase Complex cell Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure biology.protein Extracellular medicine Tyrosine Receptor 030217 neurology & neurosurgery Neurotrophin |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493937448 |
| Popis: | Receptor protein tyrosine phosphatases (RPTPs) form a group of over 20 enzymes in vertebrates, each with unique ectodomains subject to potential extracellular interactions with ligands. It has recently become clear that a remarkably diverse range of ligands exist, including homophilic binders, adhesion molecules, neurotrophin receptors, and proteoglycans. Individual RPTPs can bind several ligands, and vice versa, suggesting that complex cell signaling networks exist. The identification of RPTP ligands and where they are located in tissues remains a challenge for a large number of these enzymes. Here we describe some powerful methods that have proved successful for several research groups, leading to our improved understanding of RPTP-ligand interactions and functional regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|