Production of penicillin-specific polyclonal antibodies for a group-specific screening ELISA

Autor:	L. Okerman, Eric Cox, Bruno Goddeeris, Patricia Cliquet
Rok vydání:	2007
Předmět:	Antiserum Chromatography biology Chemistry medicine.drug_class Immunology biochemical phenomena metabolism and nutrition Monoclonal antibody Benzylpenicillin Dicloxacillin Microbiology Penicillin Antigen Polyclonal antibodies polycyclic compounds medicine biology.protein Agronomy and Crop Science Hapten Food Science medicine.drug
Zdroj:	Food and Agricultural Immunology. 18:237-252
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540100701802908
Popis:	Polyclonal penicillin-specific antibodies were obtained after the immunization of three rabbits with physiological penicillin-protein conjugates. The broad-specificity of the antisera was improved by alternate immunizations using immunogens with different penicillins as hapten. For the three antisera, the detection of ampicillin, amoxicillin, benzylpenicillin, oxacillin, cloxacillin and dicloxacillin was more sensitive in a competitive ELISA coated with the antibodies (antibody cELISA) compared to a competitive ELISA coated with a penicillin-protein conjugate (antigen cELISA). The detection of all penicillins in buffer solutions at concentrations below the MRL was achieved in the antibody cELISA when the penicillins were hydrolyzed with Penicillinase I. A simple extraction procedure was developed for the detection of penicillins in incurred porcine tissues using the antibody cELISA. Tissue fluids were used instead of the whole meat matrix, which simplified the sample preparation. Before analysis,...
Databáze:	OpenAIRE
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